APPLICATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR QUANTITATIVE ANALYSIS OF GLYCYRRHIZIC ACID IN THE COMPOSITION OF DRY EXTRACT “FLEGMEN”

Matazimov M.T.; Sidametova Z.E; Olimov N.K; Abdullaeva M.U; Khamidullaev Sh.A

APPLICATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR QUANTITATIVE ANALYSIS OF GLYCYRRHIZIC ACID IN THE COMPOSITION OF DRY EXTRACT “FLEGMEN”

Authors

Matazimov M.T. Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
Sidametova Z.E Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
Olimov N.K Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
Abdullaeva M.U Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
Khamidullaev Sh.A Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan

Keywords:

dry extract, glycyrrhizin, acid, Flegmen

Abstract

The results of determining the amount of glycyrrhizin acid in the dry extract “ Flegmen ” using high-performance liquid chromatography are presented. The composition of the dry extract " Flegmen " includes local plants - Regel's Zopnik ( Phlomisregelii M.Pop . ), Turkestan motherwort ( LeonurusturkestanicusL. ), peppermint ( Menhtapiperita L. ), naked licorice ( Glycyrrhizaglabra L.). According to pharmacopoeial article No. 42 uz-0979-2022, the biologically active substance - glycerrisin in the dry extract " Flegmen " was analyzed by highperformance liquid chromatography. It has been established that its content in 1 g of dry extract is 1.39% and corresponds to the indicator specified in the regulatory document - not less than 1%.

APPLICATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR QUANTITATIVE ANALYSIS OF GLYCYRRHIZIC ACID IN THE COMPOSITION OF DRY EXTRACT “FLEGMEN”